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  • 标题:Polygalacturonase Production by Sclerotinia Sclerotiorum, Causal Agent of Canola Stem Rot: Parameter Optimization Using Taguchi Approach
  • 本地全文:下载
  • 作者:M. Motallebi ; H. Afshari Azad ; M.R. Zamani
  • 期刊名称:World Applied Sciences Journal
  • 印刷版ISSN:1818-4952
  • 电子版ISSN:1991-6426
  • 出版年度:2008
  • 卷号:3
  • 期号:01
  • 出版社:International Digital Organization for Scientific Information Publications
  • 摘要:

    Submerged culture conditions for polygalacturonase production by Sclerotinia sclerotiorum (MR5)
    were optimized by Taguchi orthogonal array experimental design methodology. This approach facilitates the
    study of interaction of a large number of variables spanned by factors and their settings with a small number
    of experiments leading to considerable saving in time and cost for the process optimization. The objective of
    the current research was to determine the significant parameters on the production of polygalacturonase in a
    submerged culture of S. sclerotiorum. The process variables were pH, pectin as carbon source and inducer,
    incubation time and temperature. The liquid medium also included mineral salts as in Pectic Zymogram (PZ)
    medium. Polygalacturonase activity was calculated at the time of maximal extracellular enzyme activity. The
    optimal levels of the different factors for polygalacturonase production were 15 g lG it pectin, pH 5 and 4 days 1
    of incubation time at 25°C. Temperature was the most important factor in the enzyme production with 54.6%
    contribution, whereas pectin had a minimal contribution (3.19%).

  • 关键词:Polygalacturonases;Production;Sclerotinia Sclerotiorum;Causal Agents;Brassica napus;Scanning Transmission Electron Microscope;Parameters;Parameter Optimization;Approach
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