首页    期刊浏览 2022年05月21日 星期六
登录注册

文章基本信息

  • 标题:Lymph node or perineural invasion is associated with low miR-15a, miR-34c and miR-199b levels in head and neck squamous cell carcinoma
  • 作者:Lucas O. Sousa ; Lays M. Sobral ; Camila S. Matsumoto
  • 期刊名称:BBA Clinical
  • 印刷版ISSN:2214-6474
  • 出版年度:2016
  • 卷号:6
  • 页码:159-164
  • DOI:10.1016/j.bbacli.2016.11.001
  • 出版社:Elsevier B.V.
  • 摘要:Background

    MicroRNAs (miRNAs or miRs) are post-transcriptional regulators of eukaryotic cells and knowledge of differences in miR levels may provide new approaches to diagnosis and therapy.

    Methods

    The present study measured the levels of nine miRs in head and neck squamous cell carcinomas (HNSCC) and determined whether clinical pathological features are associated with differences in miR levels. SET (I2PP2A) and PTEN protein levels were also measured, since their levels can be regulated by miR-199b and miR-21, respectively. Nine miRs (miR-15a, miR-21, miR-29b, miR-34c, miR-100, miR-125b, miR-137, miR-133b and miR-199b) were measured by real time qRT-PCR in HNSCC samples from 32 patients and eight resection margins. SET (I2PP2A) and PTEN protein levels were estimated by immunohistochemistry in paired HNSCC tissues and their matched resection margins.

    Results

    In HNSCC, the presence of lymph node invasion was associated with low miR-15a, miR-34c and miR-199b levels, whereas the presence of perineural invasion was associated with low miR-199b levels. In addition, miR-21 levels were high whereas miR-100 and miR-125b levels were low in HNSCC compared to the resection margins. When HNSCC line HN12, with or without knockdown of SET, were transfected with miR-34c inhibitor or miR-34c mimic, the miR-34c inhibitor increased cell invasion capacity while miR-34c mimic decreased the cell invasion.

    Conclusions

    We showed that the levels of specific miRs in tumor tissue can provide insight into the maintenance and progression of HNSCC.

    General significance

    MiRNAs are up- or down-regulated during cancer development and progression; they can be prognosis markers and therapeutic targets in HNSCC.

  • 关键词:miR-15a ; miR-34c ; miR-199b ; Lymph node invasion ; Perineural invasion
Loading...
联系我们|关于我们|网站声明
国家哲学社会科学文献中心版权所有