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  • 标题:Cardiac Gsα overexpression enhances L-type calcium channels through an adenylyl cyclase independent pathway
  • 本地全文:下载
  • 作者:Alan S. Lader ; Yong-Fu Xiao ; Yoshihiro Ishikawa
  • 期刊名称:Proceedings of the National Academy of Sciences
  • 印刷版ISSN:0027-8424
  • 电子版ISSN:1091-6490
  • 出版年度:1998
  • 卷号:95
  • 期号:16
  • 页码:9669-9674
  • DOI:10.1073/pnas.95.16.9669
  • 语种:English
  • 出版社:The National Academy of Sciences of the United States of America
  • 摘要:The subunit of the stimulatory heterotrimeric G protein (Gs) is critical for the {beta}-adrenergic receptor activation of the cAMP messenger system. The role of Gs in regulating cardiac Ca2+ channel activity, however, remains controversial. Cultured neonatal cardiac myocytes from transgenic mice overexpressing cardiac Gs were used to assess the role of Gs on the whole-cell Ca2+ currents (ICa). Cardiac myocytes from transgenic mice had a 490% higher peak ICa compared with those of either wild-type controls or Gs-nonexpressing littermates. The effect of Gs overexpression was mimicked by intracellular dialysis of wild-type cardiac myocytes with GTP{gamma}S-activated Gs. This effect was not mediated by protein kinase A activation as intracellular perfusion with a protein kinase A inhibitor rendered the same degree of activation in either transgenic or wild-type myocytes also dialyzed with activated Gs. The data indicate that Gs overexpression is associated with a constitutive enhancement of ICa which is independent of the cAMP pathway and activation of endogenous adenylyl cyclase.
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