首页    期刊浏览 2022年12月05日 星期一
登录注册

文章基本信息

  • 标题:G1 phase arrest induced by Wilms tumor protein WT1 is abrogated by cyclin/CDK complexes
  • 本地全文:下载
  • 作者:T Kudoh ; T Ishidate ; M Moriyama
  • 期刊名称:Proceedings of the National Academy of Sciences
  • 印刷版ISSN:0027-8424
  • 电子版ISSN:1091-6490
  • 出版年度:1995
  • 卷号:92
  • 期号:10
  • 页码:4517-4521
  • DOI:10.1073/pnas.92.10.4517
  • 语种:English
  • 出版社:The National Academy of Sciences of the United States of America
  • 摘要:WT1, the Wilms tumor-suppressor gene, maps to the human chromosomal region 11p13 and encodes a transcriptional repressor, WT1, implicated in controlling normal urogenital development. Microinjection of the WT1 cDNA into quiescent cells or cells in early to mid G1 phase blocked serum-induced cell cycle progression into S phase. The activity of WT1 varied significantly depending on the presence or absence of an alternatively spliced region located upstream of the zinc finger domain. The inhibitory activity of WT1 was abrogated by the overexpression of cyclin E/CDK2 as well as cyclin D1/CDK4. Furthermore, both CDK4- and CDK2-associated kinase activities were downregulated in cells overexpressing WT1, whereas the levels of CDK4, CDK2, and cyclin D1 expression were unchanged. These findings suggest that inhibition of the activity of cyclin/CDK complexes may be involved in mediating the WT1-induced cell cycle block.
国家哲学社会科学文献中心版权所有