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  • 标题:Isolation and Characterization of Iron and Sulfur Oxidizing Bacteria from Coal Mines
  • 本地全文:下载
  • 作者:Shaiq Sultan ; Muhammad Faisal
  • 期刊名称:Journal of Environment and Earth Science
  • 印刷版ISSN:2225-0948
  • 电子版ISSN:2225-0948
  • 出版年度:2016
  • 卷号:6
  • 期号:3
  • 页码:153-157
  • 语种:English
  • 出版社:The International Institute for Science, Technology and Education (IISTE)
  • 摘要:The present study is aimed at to isolate the sulfur and iron oxidizing bacteria that can be used to remove the pollutant like as FeSO 4; MgSO 4 etc. Four bacterial isolates were isolated from coal samples called from Choa Saidan Shah, Punjab, Pakistan (Sp3 1 , Sp4 1 , Sp5 3 and Sp6 2 ) evaluated on the basis of biochemical and morphological tests. Most of the isolates showed medium sized colonies with round shape, irregular margin, creamy in color expect Sp6 2 formed yellow colored colonies. All the strains were Gram +ve rods and Spore former, Motile, Catalase producers, Starch hydrolyzing, Nitrate reducer except the SP6 2 . Strains SP3 1 and SP4 1 were sensitive against (Ampicillin-300 µg ml -1 , Tetracycline 25 µg ml -1 , Streptomycin 500 µg ml -1 , Chloramphenicol 5 µg ml -1 ) antibiotics. Maximal growth was observed at pH 7.0 except SP5 3 at pH 5.0, the optimum temperature was 42°C except SP5 3 at 37°C. After sequencing analysis as Bacillus subtilis (SP3 1 ), Bacillus subtilis (SP4) 1 , Pseudomonas sp (SP5 3 ) and Stenotrophomonas (SP6 2 ) respectively were identified. So these isolates can be exploited for bioremediation of coal.
  • 关键词:Bacillus subtilis ; Pseudomonas sp ; Stenotrophomonas; bioremediation; coal; sulfur.
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